Cloning Systems

Recombineering is a simple, rapid cloning method which allows you to directly and directionally clone your insert of choice into any linearised vector, at any site, without the need for generation of complementary ‘sticky’ DNA ends. It relies on homologous base pairing betweeen linearised vector and PCR products made using primers with sequence tags complementary to that vector at the expected ‘join site’. ***Advantages:*** - 95% cloning efficiency - Incredibly rapid - transformation-ready DNA in 15 minutes - So efficient and robust that it can be used to “stitch together” multiple PCR products and vector in one reaction