DNA Probes

DNA can be labelled by enzymatic incorporation of fluorescent nucleotide analogues. However, fine-tuning of the reaction conditions and analogue are crucial for successful labelling. Stratech supply an innovative linker technology for attaching a range of small fluorophores to nucleotides. The resulting conjugates are perfectly suited for enzymatic labelling of DNA by PCR and Nick Translation. We also provide a complete range of prelabelled nucleotides and nucleotide analogues, suitable for probe labelling, but also for studying the active site of NTP-utilising enzymes such as G-proteins. Stratech’s range of fluorophores for DNA labelling provides the following benefits: - High extinction coefficients & quantum yields - Outstanding photostability - High solubility in water - Low molecular weights - Cover the whole UV/VIS spectrum - For all common light sources and filter systems