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Glutaraldehyde

Glutaraldehyde is known to be more efficient than other aldehydes in generating thermally and chemically stable crosslinks. TAAB EM grade (G002) for use in electron microscopy has the following additional advantages: • Actual glutaraldehyde content recorded on each bottle • Buffered fixatives are stable for 6 months with pH 5 to 6. Base material viable up to 18 months and pH can be easily adjusted for use • Treatment with barium carbonate unnecessary • Low buffer requirements • Excellent fixative and is less inhibitory towards enzymes TAAB Vacuum distilled grade - purified by vacuum glass distillation to remove all polymerised material – there is no UV absorption at 235nm. It is packed in neutral glass under nitrogen for best results with enzyme histochemistry. Distilled glutaraldehyde, particularly 70% has a high risk of polymerising if it is not stored properly. It is therefore recommended that material is carefully kept at 4ºC or lower without continued defrosting and recapping. This way it can be stable for up to 18 months. Unless it is imperative that material without an absorption of 235nm is required, we strongly recommend the use of TAAB’s very effective EM grade material, eminently suitable for use in cross-linking techniques. Alcian Blue 8GX Improves preservation of intracellular substances when added to glutaraldehyde.

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